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Released N-glycan analysis of r-hGAA by PGC-HPLC-MS/MS. Extracted ion chromatograms (XICs) of the most abundant glycan structures involving different classes are as follows: oligomannose (green), hybrid (red), and complex (blue) type. P indicates the presence of a phosphoryl group on a mannose residue. MS/MS, tandem mass spectrometry; PGC, porous graphitized carbon; r-hGAA, recombinant human acid alpha-glucosidase.
