Fig. 4.

Deconvoluted mass spectrum obtained by SAX-HPLC-MS analysis of intact glycoforms of Myozyme. The four most abundant glycoforms annotated by MoFi are reported (see Supplement csv file, Intact Myozyme annotations filtered 0.01cut-off, for the complete annotation lists). The percentage indicates the fractional abundance carried by the glycoform. The third most abundant glycoform is indicated by green asterisks, and it carries the same structures of the glycoform of the mass 111842.4 Da with one sialic acid less on site N826 (sixth glycan). The second distribution of peaks (from 114 kDa to 118 kDa) is due to glycoforms where the site N869 is mainly glycosylated. MS, mass spectrometry; SAX, strong anion exchange.