Fig 7.

Pre-treatment with tert-butyl hydroperoxide enhances HCoV-OC43 viral infection. HCT-8 cells were treated with the oxidative stress inducer, tert-butyl hydroperoxide (t-BHP) or medium control 2 hours prior to infection with HCoV-OC43. Viability was measured by quantifying dehydrogenase activity in metabolically active cells (A). RNA was harvested 48 hours post-infection, and the relative abundance of spliced XBP1 (B), ERDJ4 (C), and HCoV-OC43 viral RNA (D) was determined by quantitative RT-PCR. Data are means ± SD of four replicates and are representative of two independent experiments.*P < 0.05, **P < 0.001 by unpaired t-test.