TABLE 1.
Thirty gene signatures to differentiate MDM and HAMa
| Sl no | Genes | MDM 2-hour average RPM value | HAM 2-hour average RPM value |
|---|---|---|---|
| 1 | PPARG | 56.132 | 737.089 |
| 2 | MARCO | 27.584 | 913.371 |
| 3 | DUSP1 | 72.195 | 2,810.896 |
| 4 | HBEGF | 119.366 | 2,351.293 |
| 5 | OLR1 | 49.113 | 1,881.111 |
| 6 | MRC1 | 373.582 | 1,846.700 |
| 7 | MCEMP1 | 1.958 | 1,501.346 |
| 8 | EGR1 | 9.161 | 1,220.729 |
| 9 | FOS | 1.865 | 1,214.796 |
| 10 | CES1 | 0.759 | 993.768 |
| 11 | CCL18 | 3.287 | 768.543 |
| 12 | MCL1 | 85.064 | 524.593 |
| 13 | HLA-DQA1 | 14.946 | 904.718 |
| 14 | CXCL3 | 5.762 | 783.711 |
| 15 | SERPING1 | 57.754 | 775.500 |
| 16 | ALOX5AP | 159.695 | 1,449.100 |
| 17 | CXCL5 | 4.263 | 621.929 |
| 18 | KLF4 | 2.099 | 647.161 |
| 19 | LGALS3BP | 164.032 | 934.111 |
| 20 | ALDH2 | 171.631 | 1,442.339 |
| 21 | PNPLA6 | 129.456 | 607.543 |
| 22 | MMP9 | 7,163.543 | 104.429 |
| 23 | TM4SF19 | 2,476.417 | 89.157 |
| 24 | CD84 | 930.914 | 82.818 |
| 25 | CCL22 | 2,234.179 | 66.475 |
| 26 | CD36 | 670.285 | 41.468 |
| 27 | A2M | 531.521 | 31.907 |
| 28 | MMP7 | 2,357.513 | 2.286 |
| 29 | CHIT1 | 3,702.261 | 6.979 |
| 30 | TREM2 | 808.741 | 182.914 |
a
These genes were carefully chosen based on their abundancy in HAM relative to MDM, from the literature and a previously generated AmpliSeq database from our laboratory (17, 20). Transcriptomic analysis was assessed from PBMC-derived differentiated MDM and fresh HAM after 2-hour adherence in culture. “RPM” means “reads per million,” that is, the count of how many reads map to the gene, divided by the total number of aligned reads and multiplied by one million. Lightface shows higher expression in HAM, and boldface shows higher expression in MDM.