TABLE 1.

Differences in expression of genes coding for electron transfer proteins in Geobacter metallireducens cells grown under various conditions^a

Locus ID	Annotation	Gene	Location	DIET vs Fe(III)- citrate	DIET- magnetite vs DIET	DIET vs DIET- magnetite
Gmet_0910	10 heme c-type cytochrome from PccF complex	omcF	Outer membrane/surface	6.00	2.96	NS
Gmet_0909	9 heme periplasmic c-type cytochrome from PccF complex	omaF	Periplasmic	5.32	2.76	NS
Gmet_0908	Porin protein from the PccF complex	ombF	Outer membrane/surface	5.88	2.11	NS
Gmet_0913	9 heme outer membrane c-type cytochrome from PccG	omcG	Outer membrane/surface	NS	2.78	NS
Gmet_0912	8 heme periplasmic c-type cytochrome from PccG complex	omaG	Periplasmic	NS	4.79	NS
Gmet_0911	Porin protein from the PccG complex	ombG	Outer membrane/surface	NS	3.64	NS
Gmet_1399	Type IV major pilin subunit, PilA	pilA	Outer membrane/surface	NS	NS	2.96
Gmet_1400	Short pilin chaperone protein, Spc	spc	Outer membrane/surface	NS	NS	4.29
Gmet_2928	7 heme c-type cytochrome protein from CbcABCDE	cbcA	Periplasmic	28.86	NS	NS
Gmet_2929	b-type cytochrome from CbcABCDE complex	cbcB	Inner membrane	41.70	NS	NS
Gmet_2930	Cytochrome c family protein, 11 hemes	cbcC	Periplasmic	2.55	NS	NS
Gmet_2931	Monoheme c-type cytochrome protein from CbcABCDE complex	cbcD	Periplasmic	2.00	NS	NS
Gmet_2932	Membrane protein from CbcABCDE complex	cbcE	Inner membrane	2.80	2.35	NS
Gmet_0533	Membrane protein from CbcMNOPQR	cbcQ	Inner membrane	51.56	NS	NS
Gmet_0534	c-type cytochrome protein, 5 hemes	cbcR	Periplasmic	28.19	NS	NS
Gmet_0930	c-type cytochrome, 8 hemes		Outer membrane/surface	20.45	NS	NS
Gmet_1210	c-type cytochrome, 2 hemes	ccpB	Periplasmic	5.95	NS	NS
Gmet_0252	Monoheme c-type cytochrome protein	coxB	Inner membrane	4.53	NS	NS
Gmet_1809	c-type cytochrome, 5 hemes	actA	Inner membrane	3.98	NS	NS
Gmet_1019	c-type cytochrome, 2 hemes	narC	Inner membrane	3.81	NS	NS
Gmet_2626	Monoheme P460 c-type cytochrome		Unknown	3.37	NS	NS
Gmet_0294	c-type cytochrome, 4 hemes	nrfA	Periplasmic	3.14	NS	NS
Gmet_3091	c-type cytochrome protein, 2 hemes	macA	Periplasmic	3.07	2.69	NS
Gmet_0679	c-type cytochrome, 5 hemes		Unknown	2.84	NS	NS
Gmet_1197	c-type cytochrome, 5 hemes		Unknown	2.71	3.14	NS
Gmet_0142	c-type cytochrome, 8 hemes		Periplasmic	2.44	NS	NS
Gmet_1814	Monoheme c-type cytochrome	actE	Unknown	2.30	NS	NS
Gmet_0292	Diheme c-type cytochrome		Outer membrane/surface	2.08	NS	NS
Gmet_1647	Diheme c-type cytochrome		Unknown	2.07	NS	NS
Gmet_0558	c-type cytochrome protein, 27 hemes	omcO	Unknown	NS	10.62	NS
Gmet_1087	Monoheme c-type cytochrome		Unknown	NS	7.19	NS
Gmet_0571	c-type cytochrome protein, 34 hemes		Outer membrane/surface	NS	3.23	NS
Gmet_0121	Diheme c-type cytochrome		Unknown	NS	2.89	NS
Gmet_2896	c-type cytochrome protein, 4 hemes		Outer membrane/surface	NS	2.79	NS
Gmet_0600	c-type cytochrome protein, 19 hemes		Outer membrane/surface	NS	2.67	NS
Gmet_2839	c-type cytochrome protein, 11 hemes		Periplasmic	NS	2.65	NS
Gmet_1094	Diheme c-type cytochrome	cccA	Unknown	NS	2.45	NS
Gmet_0330	Monoheme c-type cytochrome	narH	Inner membrane	NS	2.36	NS
Gmet_0557	c-type cytochrome protein, 4 hemes		Unknown	NS	2.25	NS
Gmet_0598	c-type cytochrome protein, 22 hemes		Outer membrane/surface	NS	2.15	NS
Gmet_1846	Diheme c-type cytochrome	ppcE	Periplasmic	NS	NS	3.06
Gmet_2902	Diheme c-type cytochrome	ppcA	Periplasmic	NS	NS	4.08
Gmet_3166	Diheme c-type cytochrome	ppcB	Periplasmic	NS	NS	2.75
Gmet_1867	c-type cytochrome protein, 8 hemes		Unknown	NS	NS	2.45
Gmet_2432	Monoheme P460 c-type cytochrome		Unknown	NS	NS	5.57

^a Values represent fold differences between G. metallireducens cells grown by DIET with Methanothrix thermoacetophila compared to growth with ethanol (20 mM) as the electron donor and ferric citrate (56 mM) as the electron acceptor (DIET vs Fe(III)-citrate); G. metallireducens cells grown by DIET with Mx. thermoacetophila in the presence of 10 mM magnetite versus DIET without magnetite (DIET-magnetite vs DIET); or G. metallireducens cells grown by DIET with Mx. thermoacetophila without magnetite compared to DIET with magnetite (DIET vs DIET-magnetite). Only genes with fold differences >2 and P values <0.05 were considered significant. NS: no significant difference. P values are available in Table S1.