TABLE 2.

Differences in expression of genes coding for gas vesicle proteins in Methanothrix thermoacetophila cells grown under various conditions^a

Locus ID	Annotation	Gene	Acetate vs DIET	Acetate-magnetite vs DIET-magnetite	DIET vs DIET-magnetite	Acetate vs acetate-magnetite
Mthe_0055	Gas vesicle synthesis GvpLGvpF fusion	gvpLF	2.19	1.72	2.62	3.34
Mthe_0056	Gas vesicle protein GvpO	gvpO	2.19	1.79	1.96	2.46
Mthe_0058	Gas vesicle protein GvpN	gvpN	2.27	2.05	1.71	1.88
Mthe_0060	Gas vesicle synthesis protein GvpA	gvpA	2.68	2.17	3.96	5.48
Mthe_0061	Gas vesicle synthesis protein GvpA	gvpA	2.63	2.17	4.12	5.02
Mthe_0062	Gas vesicle synthesis protein GvpA	gvpA	2.63	2.17	4.12	5.02
Mthe_0063	Gas vesicle synthesis protein GvpA	gvpA	2.63	2.17	4.12	5.02
Mthe_0069	Gas vesicle protein GvpG	gvpG	2.94	NS	4.53	11.07
Mthe_0070	Gas vesicle protein GvpA	gvpA	2.89	NS	1.94	6.25
Mthe_0071	Gas vesicle protein GvpA	gvpM	2.63	NS	2.82	8.85
Mthe_0072	Gas vesicle protein GvpK	gvpK	2.69	NS	2.40	5.71
Mthe_0073	Gas vesicle synthesis GvpLGvpF fusion	gvpLF	2.47	NS	2.06	4.72

^a Values represent fold differences between Mx. thermoacetophila cells grown by acetoclastic methanogenesis versus cells grown by DIET with Geobacter metallireducens (acetate vs DIET) as well as in the presence of 10 mM magnetite (acetate-magnetite vs DIET magnetite); Mx. thermoacetophila cells grown by DIET with G. metallireducens without magnetite compared to DIET with magnetite (DIET vs DIET-magnetite); Mx. thermoacetophila cells grown by acetoclastic methanogenesis compared to acetoclastic methanogenesis with magnetite (acetate vs. acetate-magnetite). NS, no significant difference. All P values <0.05 and are available in Table S4. DIET, direct interspecies electron transfer.