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qPCR assays evaluating the monosomic or disomic status of chromosome 1 in the FLC-sensitive and FLC-resistant strains obtained in vitro. Parental strains (A, D), FLC-resistant passage 0 strains (B, E), and passage 6 strains (C, F) were tested using primers specific for genes located on chromosome 1 right (A–C) and left arms (D–F). Primers specific to chromosome 2 were used as control.
