Fig 2.
Identification of the transcription factors regulating EV production in C. neoformans. The screening of a 155 TF mutant collection identified four major EV production regulators: HAP2, LIV4, BZP2, and GAT5 (A). Confirmation of the EV phenotype using independent knockout strains generated in the KN99α background by measuring the amount of total sterol contained in EV samples. Sterol concentration values are expressed per 109 cells for each condition (B) and single particle counting using nanoFCM (C). Cryo-electron microscopy (Cryo-EM) analysis of EVs obtained from WT and hap2Δ strains (D). Scale bars = 100 nm. Experiments were performed in at least three biological replicates. Error bars show means ± SD.