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. 2023 Jun 13;14(4):e00870-23. doi: 10.1128/mbio.00870-23

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Copyright © 2023 Rizzo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Fig 4 — Transcriptomic analysis of the EV-deficient mutants hap2Δ and gat5Δ grown in EV-producing conditions. Venn diagram revealing the number of upregulated genes in both mutants (A). Analysis of the Go-slim categories enriched in the group of genes upregulated in both mutants (B). Venn diagram revealing the number of the downregulated genes in both mutants (C). GO SLIM categories enriched in the group of downregulated genes in both mutants (D). Venn diagram revealing the overlap between genes downregulated in gat5Δ and hap2Δ, and the ones coding for the EV-enriched proteins of C. neoformans, previously identified by proteomics (E). The genes highlighted in gray with an asterisk encode proteins among the 10 most abundant proteins in C. neoformans EVs.