Fig 5.
Transcription factors regulating EV release are associated with altered azole susceptibility. Spot assay for FLC, itraconazole (Itraco), and voriconazole (Vorico) susceptibility (A) and FLC diffusion disk diffusion assay (25 µg/disk) for the four EV-defective TF mutants identified in the screening (hap2Δ, gat5Δ, liv4Δ, and bzp2Δ), and for all the members of HAP complex, including hap3Δ, hap5Δ, and hapXΔ, grown for 72 h (B). Average RAD80 values obtained from diskImageR analyses are provided. The results are illustrative of three biological replicates.