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. 2023 Jun 13;14(4):e00870-23. doi: 10.1128/mbio.00870-23

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Copyright © 2023 Rizzo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Fig 9 — Analysis of FLC susceptibility and EV production in FLC-resistant isolates obtained in vitro. Illustrative scheme of the experimental strategy used to explore the association between FLC resistance and EV production in spontaneous drug-resistant strains obtained in vitro (A). Cells at passage 0: quantitative analysis of the growth inhibition halo from the FLC-disk diffusion assays by diskImageR, RAD₈₀(B), EV production analysis by nanoFCM (C), and qualitative analysis of the inhibition halo of the FLC-disk diffusion assays (D). Cells after six successive passages in the absence of FLC: quantitative analysis of growth inhibition halo from the FLC-disk diffusion assays by diskImageR, RAD₈₀ (E), EV production analysis by nanoFCM (F), and qualitative analysis of the growth inhibition halo (G). Schematic representation created in BioRender.