FIG 3 .

hRTP4 restricts the replication of viral RNA. (A) CHME3 cells were transfected with 4 µg of hRTP4-2F expression vector or empty vector and the next day infected with HCoV-OC43 at an MOI of 0.5 (top) or 5 (bottom). Lysates were prepared at 0, 12, 36, 24, 48, and 72 h post-infection and probed for viral N protein and GAPDH. The experiment was repeated twice with similar results. (B) RNA was extracted from the infected cells, and the genomic and subgenomic viral RNAs were quantified by RT-PCR. The data are normalized to GAPDH and expressed as a fold-change relative to mock control and plotted as the means ± SD and of three biological replicates (**P < 0.01, ***P < 0.001, ****P < 0.0001).