FIG 6 .

hRTP4 binds to viral RNA. (A through C) CHME3 cells expressing the indicated hRTP4 constructs including (A) mutants ZnF1, ZnF2, and ZnF3 and a truncation TMΔ22, (B) murine RTP4, and (C) hRTP4 charged residues point mutants along with the WT hRTP4 or no FLAG-tag hRTP4 vector control were infected with HCoV-OC43 at an MOI of 5. The lysates were prepared 5 h post-infection and incubated with anti-FLAG M2 magnetic beads. The beads were then pulled-down on a magnetic and the bound virions eluted at low pH. Following elution, RNA was extracted with phenol-chloroform-isoamyl alcohol, precipitated, and analyzed by qRT-PCR for the quantification of bound genomic (top) and subgenomic (bottom) HCoV-OC43 RNAs. The data are expressed as fold-change relative to the uninfected cells. All data are the averages of three biological replicates (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).