Fig 5.

Pseudo-single cycle infectivity assays of TF virus molecular clone in A3A-to-A3G-null THP-1 cells. Infectivity of Vif-proficient and Vif-deficient CH58 viruses. Top panels show the infectivity of Vif-proficient and Vif-deficient HIV-1 produced from parental THP-1, THP-1#11-4, or THP-1#11-7 cells. The amounts of produced viruses used to infect TZM-bl cells were normalized to p24 levels. Each bar represents the average of four independent experiments with SD. Data are represented as relative to Vif-proficient HIV-1 (WT). Statistical significance was assessed using the two-sided paired t test. *P < 0.05 compared to Vif-proficient HIV-1. The bottom panels are representative western blots of three independent experiments. The levels of indicated viral and cellular proteins in viral particles and whole-cell lysates are shown. p24 and HSP90 were used as loading controls.