Fig 2.

Screening strategy for 1,4-benzodiazepines (1,4-BZDs) and control compounds. (A) Fluconazole (FLC) susceptibility profiles of Candida albicans isolates SC5314, SP-945, and P60002 on disk diffusion assays, including their mean RAD₅₀, RAD₂₀, and fraction of growth at 20% inhibition (FoG₂₀) levels. (B) A lawn of C. albicans cells was plated on YPD agar and grown in the presence of a drug disk (FLC, 25 µg) for 48 hours. The size of the radius at 50% and 20% growth inhibition (RAD₅₀ and RAD₂₀, respectively) was measured as a proxy for drug susceptibility. The FoG₂₀ was measured as a proxy for tolerance. Susceptibility and tolerance were determined using diskImageR. (C) Following 48 hours of growth, the FLC disk was replaced with a glucose disk (G, 8 mg) and the plates were grown for an additional 48 hours. For drug combinations, YPD plates contained 1,4-BZDs at 100 µM. Plates show example profiles of tolerant and resistant strains with FLC alone (left, with DMSO used as control). Plates with FLC and potentiators (right) illustrate fungistatic or fungicidal effects of different drug combinations following additional growth on glucose disks.