Fig 6.

Cytotoxicity of 1,4-benzodiazepines (1,4-BZDs) on human peripheral blood mononuclear cells (PBMCs, A). Lytic activity of the 1,4-BZDs was assessed with red blood cells (RBCs, B). PBMCs and RBCs were incubated with DMSO, PBS, Triton (positive control), or 1,4-BZDs at different concentrations, and treatment toxicity was inferred by quantifying LDH release for PBMCs (A) or by measuring hemolytic titers for RBCs (B). Assays utilized blood samples from two donors, each performed with two technical replicates, error bars show ± SEM.