Fig 3.

Heparan sulfates are required during tetraspanin-mediated staphylococcal adherence. Cells were infected with SH1000 for 60 min at an MOI = 50. (A) WT cells were treated with either 0.25 U/mL chondroitinase ABC or 0.5 U/mL heparinase I/III for 3 h prior to infection. Peptide was added to cells 60 min prior to infection for combination treatments. (B) CD9^−/− cells were treated with either 0.25 U/mL chondroitinase ABC or 0.5 U/mL heparinase I/III for 3 h prior to infection. Peptide was added to cells 60 min prior to infection for combination treatments. WT or CD9^−/− cells were treated with heparan sulfate (HS; C), dermatan sulfate (DS; D), chondroitin sulfate (CS; E), or the heparan sulfate mimetic (pixatimod; F) for 60 min prior to infection. n ≥ 3, mean ± SEM, and one-way ANOVA.