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. 2023 Aug 2;14(4):e01502-23. doi: 10.1128/mbio.01502-23

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Copyright © 2023 Alker et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Fig 4 — Marine Proteobacteria are amenable to plasmid uptake and stable replication of toolkit plasmids. (A) Maximum likelihood phylogeny built using the whole genomes of 12 strains selected for manipulation and successfully conjugated in this study (52, 53). All strains used in this study are known for their interaction with a range of marine biota and the icons depicting their associated host are shown in the vertical box. Gammaproteobacteria strains are highlighted in purple and Alphaproteobacteria strains are shown in gold. Scale bar is 0.4 and bootstraps were generated using the rapid-bootstrapping method (54). The tree was rooted at the midpoint with FigTree (v1.4.4). (B) Fluorescence and DIC overlay micrographs of overnight cultures containing constitutively expressed RSF1010 ori fluorescence vector (CP25-gfp-T7). Scale bar is 5 µm. Stars denote environmental strains that serve as the first reported conjugation for that genera.