TABLE 4.
M. kansasii essential ORFs sharing mutual orthologs,a identified as not essential in M. tuberculosis,b M. avium subsp. hominissuis,c M. abscessus,d and M. smegmatise
| Locus tag | Gene name | Description |
|---|---|---|
| MKAN_RS00320 | secA2 | Accessory Sec system translocase SecA2 |
| MKAN_RS14925 | – | Cation-translocating P-type ATPase |
| MKAN_RS16715 | – | Hypothetical protein |
| MKAN_RS17105 | thiD | Bifunctional hydroxymethylpyrimidine/phosphomethylpyrimidine kinase |
| MKAN_RS18455 | rpmG | 50S ribosomal protein L33 |
| MKAN_RS18980 | – | zf-HC2 domain-containing protein |
| MKAN_RS22360 | – | Cysteine desulfurase |
| MKAN_RS22365 | mnmA | tRNA 2-thiouridine(34) synthase MnmA |
| MKAN_RS22965 | – | DUF177 domain-containing protein |
| MKAN_RS23125 | rpsP | 30S ribosomal protein S16 |
| MKAN_RS23140 | trmD | tRNA (guanosine(37)-N1)-methyltransferase TrmD |
| MKAN_RS23630 | cobA | Uroporphyrinogen-III C-methyltransferase |
| MKAN_RS24245f | – | DUF3093 domain-containing protein |
a
Identified using Gview Server as described in Materials and Methods.
b
As defined by Dejesus et al. (35).
c
As defined by Dragset et al. (34).
d
As defined by Rifat et al. (33).
e
As defined by Dragset et al. (36).
f
Scrutiny of insertion and state call data (Data set S1) for MKAN_RS24245 revealed that three out of the six TA sites in the ORF fit the NP motif, a confounder with the potential to reduce the robustness of the ES prediction.