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SEC-MALS analysis of BxpB in Xa buffer. BxpB was separated (eluted with Xa buffer containing 2 mM TCEP) and analyzed using an analytical SEC column. The chromatogram displays UV at 280 nm (green), light scattering (red), differential refractive index (blue), and calculated molar mass (black). Nearly all BxpB (i.e., UV-absorbing material) elutes at 8.5 min with a calculated mass of 47 kDa ± 2, close to that expected for a BxpB trimer. The artifactual system peak is labeled.
