Fig 1. Volcano plots of differential gene expression at 6h Mtb infection by PMN from HITTIN and HIT.

Volcano plot for transcriptional responses to Mtb challenge for neutrophils from HITTIN (PMN_HITTIN) and HIT (PMN_HIT) participants at 6h (A-C) post Mtb infection. The y-axis shows the negative log10 unadjusted P value and the x-axis the log₂ fold change (FC). The vertical dashed lines represent log₂FC thresholds of -0.2 and 0.2. Each gene is represented by a dot. Genes which are downregulated or upregulated as determined by the FDR ≤ 5% are shown in blue and red, respectively. Genes with non-significant expression changes and below the log 2FC threshold are shown in grey. Differentially expressed genes at 6h post-infection compared to 6h uninfected PMN from HITTIN (PMN_HITTIN) (A) and HIT participants (PMN_HIT) (B). Significant differentially triggered genes between PMN_HITTIN and PMN_HIT at 6h post infection (C).