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. 2023 Sep 1;133(17):e167693. doi: 10.1172/JCI167693

Figure 8. Increased expression of phosphorylated VASP in cardiomyocytes by hypoxic RBCs.

Figure 8

(A) Expression of phosphorylated and total VASP (pVASP and tVASP, respectively) using Western blot in cardiac tissue following administration of supernatant from hypoxic (Hypo; n = 6) or normoxic (Norm; n = 6) RBCs or cGMP (0.1 mmol/L, positive control) to Langendorff-perfused mouse hearts subjected to 5 minutes ischemia and 1 minute reperfusion. (B and C) Immunofluorescence showing expression of pVASP following administration of supernatant from RBCs exposed to normoxia (n = 6 for both B and C) or hypoxia (n = 6 for both B and C). Immunoreactivity of pVASP was visualized using Alex Fluor 488 antibody (green in B) and the cardiomyocyte specific marker myosin heavy chain 7 (MYH7; red in C). Merged staining is yellow. Nuclei were stained with Hoechst (blue). Quantitative data in A and B are presented as mean ± SD. *P < 0.05 and **P < 0.01 denote significant differences by unpaired t test.