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. 2023 Sep 1;133(17):e171267. doi: 10.1172/JCI171267

Figure 1. Hepatic Mfsd2a is an early response gene and is upregulated with increasing NAFLD severity in patients.

Figure 1

(A) Feeding regimen for NASH diet–induced fatty liver in WT mice. (B) Levels of hepatic Mfsd2a expression in WT mice fed a NASH diet relative to normal chow–fed mice over time (2 weeks, n = 7; 4 weeks, n = 7; 8 weeks, n = 6; 16 weeks, n = 6; normal chow fed controls, n = 4). Mfsd2a mRNA expression was normalized to β-actin. Data are represented as the mean ratio of normalized Mfsd2a relative to Mfsd2a expression in normal chow–fed mice ± SEM. *P < 0.05; **P < 0.01, 1-way ANOVA with Dunnett’s test. (C) Hepatic Mfsd2a expression levels in individuals of normal weight (n = 14), obese individuals (n = 12), and individuals with NAFLD (n = 15) and NASH (n = 16). Normalized Mfsd2a expression was analyzed from RNA-Seq data set GSE126848. Data are expressed as normalized count. *P < 0.05, 1-way ANOVA with Tukey’s test. (D) Immunostaining of Mfsd2a in liver sections from patients without NASH (n = 5) and with NASH (n = 5). Numbers on each image correspond to patient identification numbers in Supplemental Table 1. Scale bars: 50 μm.