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. 2023 Sep 1;133(17):e171267. doi: 10.1172/JCI171267

Figure 6. Restriction of the CDP/choline pathway exacerbates features of NASH in L2aKO mice.

Figure 6

(A) Feeding regimen of mice fed on CD-NASH diet. Adult 2afl/fl and L2aKO mice were fed a CD-NASH diet for 2 weeks (n = 5 per genotype). (B) Histological analysis was performed on liver sections using H&E, α-SMA, and galectin-3. Scale bars: 50 μm. (C) Serum ALT and hepatic TGs were measured and morphometry analysis quantified the percentage areas that were positively stained for each of the indicated markers. (D) Volcano plot showing the significantly changed lipid species in L2aKO as compared with 2afl/fl mice (colored dots represent significant species and are located above dashed line, indicating a threshold of P < 0.05). (E) Graphs showing total PC, total PE, total PC containing 18:2, and total PE containing 18:2. (F) Graphs showing the specific lipid species (PC, PE, and LPC) that were significantly changed in livers of L2aKO as compared with 2afl/fl mice. Data are represented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, 2-tailed Welch’s t test.