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. Author manuscript; available in PMC: 2023 Aug 31.
Published in final edited form as: Int J Dev Biol. 2014;58(10-12):799–809. doi: 10.1387/ijdb.140215ml

Fig. 5. In situ hybridization for collagen9a2 confirms the microarray result of left sided expression prior to nodal flow.

Fig. 5.

(A) Embryos processed by in situ hybridization for the marker col9a2 show expression only on the left side or (B) on both sides but with a strong left bias. Embryos were examined prior to the development of ciliary flow. (C) Control embryos probed for the marker Xslug, which was not biased in the microarray results, showed bilateral symmetric expression in all animals examined. Red arrows indicate areas of expression; white arrows indicate absence of expression. The dotted line demarcates the embryo’s midline. All panels show a dorsal view, with the anterior pointing upward.