Table 1.
PHARMACOLOGICAL REAGENTS TESTED IN SINGLETON EMBRYOSa
| Treatment | Target | Exposure period |
|||||
|---|---|---|---|---|---|---|---|
| 1 cell - neurula |
St 8 - neurula |
||||||
| n | % heterotaxia | % inverted hearts | n | % heterotaxia | % inverted hearts | ||
|
| |||||||
| Lindane (2μM) | GJC | 116 | 21 | 12 | 237 | 1 | 0 |
| Tropisetron (10μM) | 5-HT | 84 | 23 | 16 | 150 | 3 | 2 |
| BaCl (10mM) | k+ | 82 | 23 | 16 | 127 | 3 | 1 |
| pH 4.0 | h+ | 247 | 32 | 24 | 202 | 3 | 2 |
| Fluoxetine (0.8μM) | 5-HT | 84 | 36 | 25 | 79 | 1 | 0 |
a
These reagents were tested in singleton embryos either early (1 cell - neurula) or late (stage 8 - neurula), to identify reagents that could be used In conjoined twins.