Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 27;26(9):1516–1528. doi: 10.1038/s41593-023-01396-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — a, Left, coronal plane schematics highlighting the PPN (magenta) at different rostro-caudal levels. Right, confocal photomicrographs showing the distribution of Chx10⁺ (green, Chx10^Cre; R26^EYFP reporter mouse) and ChAT⁺ (magenta, antibody) neurons at the corresponding rostro-caudal levels of the schematics to the left. The pictures at the right-most column are magnified images corresponding to the area delineated by dashed lines in the pictures to the left. The same area is also delineated in the schematics with orange dashed lines. White arrowheads in the magnified pictures point to the PPN. Chx10⁺ neurons are sparse within the caudal half of the PPN while their density increases in the rostral half of the PPN, following an inverted pattern compared to ChAT⁺ neurons within the nucleus. There is no overlap in the cellular expression of ChAT and Chx10 at any rostro-caudal level of the PPN. Chx10⁺ neurons are also found in the vlPAG where they are enriched at the coronal levels corresponding to the caudal half of the PPN (see also Extended Data Fig. 8).