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. 2023 Aug 31;14(8):578. doi: 10.1038/s41419-023-06105-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Schematic diagram of functions of circRNA-translated proteins. B The expression levels of MIB2 and circMIB2 in spleen samples were measured by qPCR at the indicated time after SCRV infection and V. anguillarum stimulation. C Confirmed the head-to-tail splicing of circMIB2 in the circMIB2 PCR product by Sanger sequencing. D, E The expression of circMIB2 and linear MIB2 mRNA in both MSpC and MIC cell lines was detected by PCR assay followed by nucleic acid electrophoresis assay in the presence or absence of RNase R. F Detect the distribution and proportion of MIB2 mRNA and circMIB2 in MIC and MSpC cells through RNA FISH assay. All data represented the three independent triplicated experiments. **p < 0.01.