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. 2023 Aug 31;13:14323. doi: 10.1038/s41598-023-41334-8

Table 1.

Soft agar overlay assay.

Strains IHD (mm) SD (mm) R AU/ml
L. curvatus CRL705 C = 16 C = 10 3 L 800
P = 18 P = 8 5 L 900
S = 0 S = 0 WI 0
L. mucosae CRL573 C = 16 C = 11 2.5 L 800
P = 16 P = 9 3.5 L 800
S = 0 S = 0 WI 0
L. acidophilus CRL641 C = 0 C = 11 WI 0
P = 15 P = 9 3 L 750
S = 0 S = 0 WI 0
F. tropaeolis CRL2034 C = 12 C = 10 WI 600
P = 12 P = 9 WI 600
S = 0 S = 0 WI 0
L. reuteri CRL1101 C = 7 C = 6 WI 350
P = 16 P = 9 3.5 L 800
S = 0 S = 0 WI 0
C. farciminis CRL748 C = 0 C = 8 WI 0
P = 15 P = 8 3.5 L 750
S = 0 S = 8 WI 0
Lcb. rhamnosus ATCC53103 C = 0 C = 8 WI 0
P = 12 P = 8 WI 600
S = 0 S = 8 WI 0
Lcb. rhamnosus CRL75 C = 15 C = 11 2 L 750
P = 15 P = 8 3.5 H 750
S = 6 S = 6 WI 300
Lcb. rhamnosus Principia C = 10 C = 8 WI 500
P = 14 P = 8 3 L 700
S = 0 S = 8 WI 0
Lcb. rhamnosus CRL 2244 C = 32 C = 9 (18) 7 H 1600
P = 33 P = 8 (18) 7.5 H 1650
S = 0 S = 6 WI 0

Antimicrobial activity of culture (C), pellet (P) and supernatant (S) spot of LAB on A. baumannii A118 at 16 h incubation. Inhibition halos diameter (IHD) were measured, and inhibitory capacity was interpreted > 20 mm = High (H), 20–10 mm = Intermediate (I) and < 10 mm = Low (L). The width of the clear zone (R) is the difference between IHD and spot diameter divided by two (SD), inhibitory capacity was interpreted as R > 6 mm = High (H), 5–2 mm = Low (L), < 2 mm = Without Inhibition (WI). The arbitrary units per ml (AU/ml) was calculated as the IHD × 1000 divided by the μl seed (Halder et al.5). The average of three independent assays is represented on the table.