Fig. 1.

Experimental paradigm. Eker rat pups were exposed to VEH and EDC diethylstilbestrol at postnatal days 10-12. The pups were sacrificed at 5 months of age representing the early adult stage. Myometrial tissues were isolated from the animals and subjected to MMSC isolation using Stro-1/CD44 surface markers. Myometrium from five animals was pooled for each treatment. Multi-omics analyses, including RNA-sequencing, ChIP-sequencing, and RRBS, were performed to determine the global alterations of the transcriptome, histone modification, and DNA methylation, respectively. In addition, targeted bisulfite NGS was performed to examine the DNA methylation within CpG islands of genes