Fig. 4.

Developmental exposure to EDC activates MLL1. a Western blotting with anti-MLL1, -H3K4me3, and -Cd9 antibodies was performed to determine the levels of MLL1C (the activated form of MLL1), H3K4me3 and CD9. Total H3 and β-actin were used as loading controls. Biological replicates (n = 3) were used for quantification. b Confocal imaging showing the staining of H3K4me3 in EDC- vs. VEH-MMSCs and quantitative analysis. Three separate coverslips of cells were used for quantification. Student’s t-test, *p < 0.05; **p < 0.01; ***p < 0.001