Fig. 6.

Knockdown of Tasp1 reversed the reprogrammed ERGs induced by EDC exposure. a Western blot analysis was conducted to determine the role of TASP1 in MLL1-mediated epigenetic pathways after knock-down of Tasp1 expression using shRNA lentiviral (pLKD-TASP1) plasmids in EDC-MMSCs. The levels of expression of TASP1, H3K4me3, and CD9 proteins were measured by Western blot analysis using antibodies against TASP1, H3K4me3 and CD9, respectively (left panel). Biological replicates (n = 3) were used for quantification. Quantitative analysis was performed using Image J (right panel). b the upregulation of the expression of 6 reprogrammed ERGs (Esr-1, Pgr, Cxcl12, Ar, Cd9, and Tgm2) induced by EDC exposure was reversed by Tasp1 knock-down in EDC-MMSCs. RNA expression of ERGs was measured by qPCR in EDC-MMSCs infected with either scrambled lentivirus or one of three different individual Tasp1 knock-down lentiviruses. *p < 0.05; **p < 0.01; ***p < 0.001; Student’s t-test