Fig. 8.

Specific reprogramming of ERGs in DES-MMSCs, which impacts DMC. a Bar plots showing the differential expression of ERGs, including Bcl11b, Cd9, Cxcl12, and Tgm2, between DES-MMSCs and DES-DMCs. b The correlation between H3K4me3 status and compared expressions of reprogrammed ERGs between MMSCs and DMCs using VEH-MMSC as a reference. The p-value shows the significant difference in gene expression between Stro-1/CD44 double-positive and double-negative cells. The genes with light blue background indicate that two comparisons of the gene expression (Stro-1/CD44 double-positive vs. double-negative cells, or Stro-1⁺/CD44⁺ DES vs. VEH) going in the opposite direction. The genes with white background indicate that two comparisons go in the same direction. c Diagram of experimental design. Serum-free conditioned medium (CM) was prepared from EDC-MMSCs and VEH-MMSCs. DMCs from rat adult myometrium were grown in the CM from EDC-MMSCs and VEH-MMSCs for 2 days. d MTT assays were performed to measure DMC proliferation in the presence of CM from either EDC-MMSCs or VEH-MMSCs. e qPCR was performed to determine the effect of CM from EDC-MMSCs and VEH-MMSCs on the expression of β-catenin and β-catenin-regulated genes (Angpt2, Med12l, and Pitx2) in DMCs. *p < 0.05; **p < 0.01, ****p < 0.0001