Fig. 1.

Effects of G6PDi-1, 6AN and DHEA on the activity of G6PDH in lysates of cultured astrocytes. The activity of the G6PDH was determined by the increase in NADPH absorbance at 340 nm in the absence or the presences of the indicated inhibitors. Panel a shows values from one representative experiment for 10 µM of the listed inhibitors. The linear increase in absorbance per minute was used to calculate the activity of G6PDH. Panel b shows the G6PDH activities calculated for the given concentrations of inhibitors. Half-maximal inhibition of G6PDH was found for 102 ± 3 nM of G6PDi-1 and for 9.2 ± 3.5 µM of DHEA. The protein content of the supernatant was 439 ± 19 µg per dish. The values in panel a are from one representative experiment that was reproduced twice with comparable results in independent experiments on lysates from other cultures. The data shown in panel b are means ± SD of values from independent experiments that had been performed on three independently prepared astrocyte cultures