Fig. 2.

Test for potential effects of G6PDi-1 on the glucose consumption, the lactate release, the glutathione metabolism and the viability of cultured astrocytes. The cells were incubated without (0 µM) or with the given concentrations of G6PDi-1 in glucose-containing (5 mM) incubation buffer for up to 6 h. Glucose consumption (a), lactate release (b), cellular (c) and extracellular (e) GSx contents, cellular (d) and extracellular (f, g) GSSG contents as well as extracellular LDH activity (h) were measured for the indicated time points. The initial specific GSx content was 35.4 ± 5.6 nmol/mg protein and the specific GSSG content was 0.6 ± 0.1 nmol GSx/mg protein. The initial protein content of the cultures was 145 ± 15 µg per well. The data presented are means ± SD of values from experiments that had been performed on three independently prepared astrocyte cultures. The significance of differences (as calculated by ANOVA) compared with data for incubations without G6PDi-1 (0 µM) is indicated by ***p < 0.001