Table 2.

Effects of G6PDi-1 and the NQO1 inhibitor ES936 on the β-lapachone-mediated WST1 reduction by astrocytes

	LDH	WST1 formazan content
Condition	(%)	(nmol/mg protein)
No inhibitor	2 ± 2	332 ± 56
100 µM G6PDi-1	3 ± 1	165 ± 13***
3 µM ES936	3 ± 3	37 ± 1***
100 µM G6PDi-1 + 3 µM ES936	2 ± 1	17 ± 2***

The cells were preincubated for 30 min in the absence of glucose and subsequently incubated for 60 min with 5 mM glucose, 400 µM WST1 and 3 µM β-lapachone in the absence (0 µM) or the presence of the indicated inhibitors. After 60 min incubation, the extracellular WST1 formazan content and the extracellular LDH activity (given as percent of the initial cellular LDH activity) were measured. The data listed for the control (No inhibitor) and for the 100 µM G6PDi-1 treatment are taken from Fig. 3. The initial protein content of the cultures was 154 ± 16 µg per well. The data presented are means ± SD of values from experiments that had been performed on three independently prepared astrocyte cultures. The significance of differences (as calculated by ANOVA) compared with data for incubations without inhibitor is indicated by ***p < 0.001