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. 2023 Aug 18;14:1239133. doi: 10.3389/fpls.2023.1239133

Figure 4.

Figure 4

Direct conversion of root primordia to shoot meristems. High auxin concentration triggers the formation of a competent lateral root (CLR). After the transfer to cytokinin rich medium (dashed black line), cell division is attenuated in the paused competent lateral root (pCLR). The cell cycle is paused for 24 hours and expression of root-specific WOX5 is terminated. The activity of other root identity markers lasts for 24 hours after transfer for PLT2 and SHR, and 63 hours for SCR. The shoot identity markers WUS and CLV3 are detected after 19 and 32 hours, respectively. 48 hours after transfer to cytokinin rich medium, conversion of meristematic identity takes place in the converting organ (CO). 120 hours after transfer, a shoot apical meristem (SAM) is formed.