Table 1.

All the primers and plasmids used in this study are listed in table.

Strain	Relevant genotype	Source
C. freundii ESBL	Clinical isolate	Hospital
ΔAmpR	C. freundii AmpR mutant	This study
E. coli DH5α	Transformation	TransGen
E. coli BL21	Protein expression	TransGen
Plasmids
pKD4	Template for FLP recognition target site, Kanr, Ampr	[26]
pKD46	Temperature-sensitive, λ Red recombinase plasmid, Kanr, Ampr	[26]
pCP20	Temperature-sensitive, FLP recombinase plasmid, Ampr, Cmr	[26]
pET28a (+)	Recombinant protein expression in E. coli BL21, Kanr	Novagen
Primer name	Oligonucleotide (5′-3′)	Application
AmpR- forward-1	AGGCTTAATGATGACGCGTAGCTATATCCCTCTGTAGGCTGGAGCTGCTTC	AmpR mutant
AmpR-reversed-2	GTTAATTATCAGGCCCGCCATTGGCGGGCCTTTTCATATGAATATCCTCCTTAG	AmpR mutant
AmpR-forward-3	ATGACGCGTAGCTATATCCCTCTTAAC	PCR
AmpR-reversed-4	GTGCAGCACCCCGGTCAACCAACGGGA	PCR
AmpR-forward-rt1	GTGACGCATTCTGCCATCAGCCAGC	RT-qPCR
AmpR-reversed-rt2	TTCCTGGGTCTGTTTAGTGGCAA	RT-qPCR
AmpC-forward-7	TGATTTCATGATGAAAAAATCGATATGC	PCR
AmpC-reversed-8	CAGTTATTGCAGTTTTTCAAGAATGCGC	PCR
AmpC-forward-rt3	GAGCAGGCTATTCCGGGCATGGCCG	RT-qPCR
AmpC-reversed-rt4	ATAGCGTCGCCGCCCAACACGCCGT	RT-qPCR