Table 2.
The API 20E test results of ESBL C. freundii strain.
| S. No. | Test (Enzyme/Substrate) | Negative reaction | Positive reaction | C. freundii results |
|---|---|---|---|---|
| 1. | O-nitrophenyl-beta-d-galactopyranoside (ONPG) | Colorless | Yellow | + |
| 2. | Arginine dihydrolase (ADH) | Yellow | Red or orange | + |
| 3. | Lysine decarboxylase (LDC) | Yellow | Red or orange | – |
| 4. | Ornithine decarboxylase (ODC) | Yellow | Red or orange | – |
| 5. | Citrate utilization (CIT) | Pale to green | Blue green | + |
| 6. | Hydrogen sulfide (H2S) | Colorless | Black deposits | + |
| 7. | Urease (URE) | Yellow | Red or orange | – |
| 8. | Tryptophan deaminase (TDA) | Pale yellow or brownish | Brown red | _ |
| 9. | Indole (IND) | Pale yellow or colorless | Red (within 2-mints) | _ |
| 10. | Voges–Proskauer (VP) | Pale yellow or colorless | Red or pink (within 10-mints) | _ |
| 11. | Gelatinase (GEL) | No black diffusion | Black diffusion | – |
| 12. | Glucose (GLU) | Blue or blue-green | Red or yellow | + |
| 13. | Mannitol (MAN) | Blue or blue-green | Red or yellow | + |
| 14. | Inositol (INO) | Blue | Yellow | – |
| 15. | Sorbitol (SOR) | Blue green | Yellow | + |
| 16. | Rhamnose (RHA) | Blue | Yellow | + |
| 17. | Sucrose fermentation (SAC) | Blue | Yellow | + |
| 18. | Melibiose (MEL) | Blue green | Yellow | + |
| 19. | Amygdalin (AMY) | Blue | Yellow | – |
| 20. | Arabinose (ARA) | Blue | Yellow | + |