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. 2023 Jun 16;29(17):3498–3513. doi: 10.1158/1078-0432.CCR-23-0219

Figure 4.

Figure 4. Infiltrating macrophages within immunosuppressive peritumoral niches undergo Activin A–mediated transcriptional reprogramming. A, Subclustering of the Myeloid cells shown as a UMAP plot (left) and a dot plot of the average expression level of the myeloid marker genes (right). B, Color scale overlaid UMAP plot of the average expression level of the difference between STME2 and STME1 signature scores in the Macrophage population. C, Clusters ranked according to the average expression level of the difference between STME2 and STME1 signature scores in the Macrophage population, represented as a UMAP plot (left) and as violin plots (right). D, Color scale overlaid UMAP plot of the average expression level of the genes present in the Activin A exposure signature in the Macrophage population. E, Violin plots of the clusters ranked according to the average expression level of the genes present in the Activin A exposure signature in the Macrophage population. F, Representative images of a CD163 (green) and DAPI (blue) costaining with INHBA (red) and LAIR1 (orange) RNA FISH probes on a human BCC within previously identified FAPlow/CD8high versus FAPhigh/CD8low areas. Scale bar, 50 μm.

Infiltrating macrophages within immunosuppressive peritumoral niches undergo Activin A–mediated transcriptional reprogramming. A, Subclustering of the Myeloid cells shown as a UMAP plot (left) and a dot plot of the average expression level of the myeloid marker genes (right). B, Color scale overlaid UMAP plot of the average expression level of the difference between STME2 and STME1 signature scores in the Macrophage population. C, Clusters ranked according to the average expression level of the difference between STME2 and STME1 signature scores in the Macrophage population, represented as a UMAP plot (left) and as violin plots (right). D, Color scale overlaid UMAP plot of the average expression level of the genes present in the Activin A exposure signature in the Macrophage population. E, Violin plots of the clusters ranked according to the average expression level of the genes present in the Activin A exposure signature in the Macrophage population. F, Representative images of a CD163 (green) and DAPI (blue) costaining with INHBA (red) and LAIR1 (orange) RNA FISH probes on a human BCC within previously identified FAPlow/CD8high versus FAPhigh/CD8low areas. Scale bar, 50 μm.