Fig. 4.

Mcl-1 deficiency leads to prolonged SAC activation, mitotic errors and chromosome mis-segregation in steady-state livers of 2-month-old mice.

(A, B) GSEA comparing all differentially regulated genes from livers of 2-month-old Mcl-1^Δhep mice with various gene sets. (C) Table of the 10 most enriched genes involved in the regulation of metaphase obtained by GSEA analysis of 2-months-old WT vs. Mcl-1^Δhep mice. (D). Representative images of Hoechst and pHH3 staining of livers of 2-month-old WT mice displaying normal prophase nucleus and symmetric bipolar metaphase plate. Representative images of Mcl-1^Δhep mice displaying (E) normal and (F) abnormal mitotic figures with spindle multipolarity and asymmetry, (G) chromosome exclusion as well as (H) lagging and bridging chromosomes. (I) Percentage of aberrant mitotic figures relative to normal mitotic figures in Mcl-1^Δhep livers. GSEA, gene set enrichment analysis; Mcl-1, myeloid cell leukaemia sequence 1; pHH3, phospho-histone H3; SAC, spindle assembly checkpoint; WT, wild type.