Fig. 5.

Chromosomal instability, heterogeneous genetic mutations but consistent mutational signatures are observed in Mcl-1^Δhep tumours of 12-month-old mice.

(A) WES was performed on DNA isolated from 25 discrete neoplasms excised from the livers of 15 Mcl-1^Δhep mice. As controls, DNA from four non-tumoral parts of Mcl-1^Δhep livers and four WT livers was isolated. (B) Number of SNV deletions in the coding regions per sample of Mcl-1^Δhep DN and HCC (n = 13). (C) Percentage of the mean SNV and indel derived chromosomal instability in normal tissues of WT mice (n = 4) and in tumoral tissues of Mcl-1^Δhep HCC (n = 13). ∗∗p ≤0.01. (D, E) SBSs and small ID COSMIC signatures of Mcl-1^Δhep DN and HCC samples (n = 26). DN, dyplastic nodule; COSMIC, catalogue of somatic mutations in cancer; HCC, hepatocellular carcinoma; ID, insertion and deletion; Mcl-1, myeloid cell leukaemia 1; SBS, single-base substitution; SNV, single nucleotide variation; WES, whole exome sequencing; WT, wild type.