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. 2023 Aug 19;66:102856. doi: 10.1016/j.redox.2023.102856

Fig. 8.

Fig. 8

Replacing Cys319 with Ser (CRISPR) [10] increases the EC50 of CP-8.A. Cells were counted every 4 h for 6 days. K values of the logistic growth curve are plotted and analyzed by Two-way ANOVA. B. increases the EC50 of CP-8 in MM231 cells in a 3 d CellTiterGlo assay. OA = oleic acid, a non-nitrated nitroalkene control. C. TNBC organoids were treated daily with 5 μM and 10 μM CP-8 for 3 days. Viability was assessed with CellTiter Glo3D (relative luminescence), and data were normalized to vehicle (DMSO) treated cells (left). Bright-field microscopy images showing representative phenotype of treated PDOs (right). Average ± SEM n = 3.