Table 2.

Primer sequences of quantitative real-time PCR.

Gene name	Primer sequences (5′–3′)	Accession no.
β-Actin	F: TCTGGCACCACACCTTCT R: TGATCTGGGTCATCTTCTCAC	XM_021086047.1
Occludin	F: AACGTATTTATGACGAGCAGCCC R: CACTTTCCCGTTGGACGAGTA	XM_005672525.3
ZO-1	F: AAGGTCTGCCGAGACAACAG R: TCACAGTGTGGTAAGCGCAG	XM_021098896.1
MUC2	F: GGACGACACCATCTACCTCAC R: AGGCCAGCTCGGGAATAGA	XM_021082584.1
TNF-α	F: TCTGCCTACTGCACTTCGAG R: GTTGATGCTCAAGGGGCCA	NM_214022.1
IL-8	F: GACTTCCAAACTGGCTGTTGC R: ATTTGGGGTGGAAAGGTGTG	NM_213867.1
IL-4	F: CCCGAGTGTCAAGTGGCTTA R: TGATGATGCCGAAATAGCAG	NM_214340.1
IL-1β	F: CCAAAGAGGGACATGGAGAA R: GGGCTTTTGTTCTGCTTGAG	NM_001302388.2
IFN-γ	F: CAGCTTTGCGTGACTTTGTG R: GATGAGTTCACTGATGGCTTT	NM_213948.1
NF-κB	F: GACCTGGTTTCGCTCTTG R: TGCTGTATCCGGGTACTT	NM_001114281.1
P38 MAPK	F: ATTCTCCGACGGTCTCAAGT R: GCCACATAGCCTGTCATT	XM_021079285.1
NF-κB p65	F: TCATCGAGCAGCCCAAGCA R: CAGCCTCATAGAAGCCATCCC	NM_001114281.1
IκBα	F: CACCCGAGTTAGAAGGGCTC R: GGTATCTGCTGAGGTGTGCTG	XM_001924394.6

β-Actin = β-non-muscle actin; ZO-1 = zonula occludens-1; Muc 2 = mucin 2; TNF-α = tumor necrosis factor-α; IL-8/4/1β = interleukin-8/4/1β; IFN-γ = interferon-γ; NF-κB = nuclear factor-k-gene binding; p38 MAPK = p38 mitogen-activated protein kinase; NF-κB p65 = nuclear factor-κB p65; IκBα = inhibitor of κB alpha.