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. 2023 Mar 7;56(9):e13440. doi: 10.1111/cpr.13440

FIGURE 4.

FIGURE 4

Piezo1 is required for the mechanically induced M2 polarization. (A) mRNA levels of calcium channels in RAW264.7 cells were determined by RT‐PCR. (B) Differential signalling pathways were analysed from the database (GSE139121). (C) Immunofluorescent staining of acetyl‐p53 (red) in RAW264.7 cells under the tension of 0–6 h. Blue indicates DAPI staining of nuclei. Scale bar: 10 μm. (D) Western blot was used to identify the p53 and acetyl‐p53 protein expression in RAW264.7 cells as a result of 0–6 h of tension. GAPDH was used for normalization. (E) In the control or Piezo1 knock‐down groups, p53 and acetyl‐p53 protein expression were tested under static or tension conditions. (F) Piezo1, Ptgs2, Socs3, Cxcl10, Arg‐1, MRC1, Edn1, IL‐10 and TGF‐β1 were detected by qPCR in control or Piezo1 knock‐down groups that had been cultivated under tension for 2 h. (G) Western blotting was used to identify the protein expression of iNOS and Arg‐1 in three groups (the control group, the Piezo1‐knockdown group and the Yoda1‐administered group) during 2 h of tension. Data represent the mean ± SD. *p < 0.05, ** p < 0.01, ***p < 0.001.