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. 2023 Sep 1;18:53. doi: 10.1186/s13062-023-00409-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Upregulated METTL3 increases GBAP1 expression in HCC. A RT-qPCR analysis was applied to explore METTL3 expression in HCC tissues (n = 85) and adjacent non-tumor tissues (n = 85) (mean ± SD; n = 3). ***P < 0.001 (Student’s t test). B Data from TCGA showed that METTL3 expression was significantly increased in HCC tissues (n = 371) compared to normal tissues (n = 50) (https://ualcan.path.uab.edu/index.html) (Student’s t test). C RT-qPCR analysis was applied to explore METTL3 expression in HCC cell lines and normal hepatic cell LO2. ***P < 0.001 versus LO2 (two-way ANOVA). D Data from UALCAN platform showed that HCC patients with higher METTL3 expression had worse prognosis than that with lower METTL3 (https://ualcan.path.uab.edu). E and F RT-qPCR analysis and Western blot were applied to verify the overexpressing efficiency of pcDNA/METTL3 in Hep3B cells. ***P < 0.001 (Student’s t test). G and H RT-qPCR analysis and Western blot were applied to verify the knockdown efficiency of METTL3 shRNA#1 and shRNA#2 in MHCC97H cells. ***P < 0.001 versus shNTC (two-way ANOVA). I RNA-seq was conducted in METTL3 overexpressing and the control sunclones of Hep3B. Scatter diagram was applied to show the differentially expressed genes which were regulated by METTL3 in Hep3B cells. J RT-qPCR analysis was applied to explore GBAP1 expression in HCC tissues (n = 85) and adjacent non-tumor tissues (n = 85). ***P < 0.001 (Student’s t test). K RT-qPCR analysis was applied to explore GBAP1 expression in HCC cell lines and normal hepatic cell LO2. ***P < 0.001 versus LO2 (two-way ANOVA). L Pearson correlation analysis showed that there existed a positive correlation between METTL3 and GBAP1 in HCC tissues (n = 85). M and N RT-qPCR analysis showed that GBAP1 was significantly increased by pcDNA/METTL3 in Hep3B cells, while GBAP1 was significantly decreased by METTL3 shRNAs. ***P < 0.001 (Student’s t test). ***P < 0.001 versus shNTC (two-way ANOVA)