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. 2023 Aug 31;28(1):2251237. doi: 10.1080/13510002.2023.2251237

Figure 4.

Figure 4.

Treatment with LIPUS inhibited the expression of fibrin and fibrosis factor in the ADR-induced CKD rat. (A) The expression levels of fibronectin, collagen I and collagen III in rat kidney tissue were assayed by immunofluorescence. Scale bar, 50 μm. The absolute expression levels of fibronectin (B), collagen I (C) and collagen III (D) were quantified as the average fluorescence intensity. (E) The protein expression levels of E-cadherin and α-smooth muscle actin (α-SMA) in rat kidney tissue were assayed by western blotting (WB). Relative expression levels of E-cadherin (F) and α-SMA (G) in each group. The mRNA expression levels of fibronectin (H), E-cadherin (I) and α-SMA (J) in rat kidney tissue were proved by real-time quantitative polymerase chain reaction (RT-qPCR). Data represent the mean ± standard error of the mean (SEM) for 6 rats in each group. *p < 0.05, ** p < 0.01, ***p < 0.001, **** p < 0.0001.