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. 2023 Aug 31;28(1):2251237. doi: 10.1080/13510002.2023.2251237

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 5. — Treatment with LIPUS improved iron deposition and apoptosis in the ADR-induced CKD rat. (A) Representative micrographs of iron staining in each group. Scale bar, 50 μm. (D) Quantification of iron deposition area, expressed as a percentage of the total area. (E) The levels of Fe²⁺ in rat kidney tissue were assayed by enzyme linked immunosorbent assay (ELISA). (B) The protein expression level of ferritin heavy chain 1 (FTH1) in rat kidney tissue was assayed by western blotting (WB). (F) Relative expression levels of FTH1. (C, G) Representative micrographs of TUNEL staining and result of apoptosis analysis in each group. Data represent the mean ± standard error of the mean (SEM) for 6 rats in each group. *p < 0.05, ** p < 0.01, **** p < 0.0001.