Fig. 2.

CNS neurotoxic effects of 2,4,5-T. Human PNET2 CNS cells seeded into 96-well plates were treated with 0, 25μg/ml, or 250μg/ml of 2,4,5-T for 48 h. A) Absorbance for MTT activity (540 nM) reflecting mitochondrial function. B) Hoechst H33342 fluorescence (Ex360/Em460) reflecting viable cell number. N = 16 culture well replicates per treatment group. Graphs depict individual sample data with means (horizontal bars) and standard deviations (stems). Significant post hoc multiple comparison Tukey test results are displayed.