Fig. 4.

Metabolic and oxidative stress indices modulated by Agent Orange herbicide toxins. Human CNS-derived PNET2 cells were treated for 48 h with Vehicle, 250μg/ml 2,4,5-T, or 250μg/ml of 2,4-D. (A) GAPDH, (B) 8-OHdG, and (C) HNE immunoreactivity were measured by duplex ELISA with results normalized to RPLPO. Each group included 6 replicate cultures. Results were analyzed by one-way ANOVA with post hoc Tukey tests (repeated measures; See Table 5). Significant p-values are indicated within the graphs. 2,4,5-T, 2,4,5-trichlorophenoxyacetic acid; 2,4-D, 2,4-dichlorophenoxyacetic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; 8-OHdG, 8-hydroxydeoxyguanosine; HNE, 4-hydroxy-2- nonenal; RPLPO, Large acidic ribosomal protein.